Revealing alkali ion modulation on the nucleation phase of highly concentrated α-synuclein probed by Thioflavin T lasing spectroscopy
Early nucleation events during α-synuclein (αS) aggregation remain challenging to investigate because conventional fluorescence assays lose sensitivity in highly concentrated biomolecular environments. Here, we present Thioflavin T (ThT) lasing spectroscopy in a Fabry–Pérot microcavity as a sensitive methodological app...