A NanoLuc-Based Protease Biosensor for Highly Sensitive Detection of Intracellular Protease Activity: Applications to Apoptosis and Coronavirus Infection
FlipNanoLuc is a highly sensitive protease biosensor based on the β-strand-flipping principle of NanoLuc luciferase, which is derived from Oplophorus gracilirostris. In the inactive configuration, one β-strand of NanoLuc is repositioned, thereby suppressing luciferase activity. Upon cleavage of the embedded protease re...