Mechanistic Insights into Magnesium Pyrophosphate Formation in the Presence of Gold Nanoclusters Enable Genetic Analysis via Co-Aggregation-Induced Fluorescence Enhancement
The formation of magnesium pyrophosphate (Mg2P2O7) in nucleic acid amplification and cell-free transcription systems has attracted considerable attention, since Mg2P2O7 serves as a reliable indicator of reaction efficiency. However, real-time monitoring of Mg2P2O7 remains challenging, relying largely on time-consuming...