Functional expression of engineered IsPETase and LCC variants in Drosophila melanogaster reveals host tolerance and differential catalytic activity.
Insects offer promising opportunities for organic waste bioconversion; however, they cannot efficiently degrade synthetic polymers such as polyethylene terephthalate (PET). Here, we generated transgenic Drosophila melanogaster lines to express in vitro-evolved variants of two PET-degrading enzymes with distinct biochem...