Evan R. Williams

Navigating the oligomeric landscape of the periplasmic stress response protease-chaperone DegP with charge detection mass spectrometry

Charge detection mass spectrometry (CDMS) is applied to directly measure the masses of individual DegP ions, resolving the complete oligomeric distribution in the absence and presence of four clients of increasing size and further establishes CDMS as a powerful single-molecule tool for dissecting heterogeneous protein...