Systematic Evaluation of Mesophyll Protoplast Isolation and PEG-Mediated Transient Expression in Rosa chinensis ‘Old Blush’
Stable transformation in rose is slow and genotype-dependent, motivating rapid cellular assays for candidate-gene testing. We systematically evaluated mesophyll protoplast isolation and PEG-mediated transient expression in Rosa chinensis ‘Old Blush’. Sequential experiments compared enzyme preparations, digestion time,...