A rapid isocratic LC-MS/MS method for the quantification of busulfan in human plasma and its clinical application in pediatric bone marrow transplantation.
A rapid isocratic LC-MS/MS method for quantifying busulfan in human plasma was developed and validated. Plasma samples were processed by protein precipitation with acetonitrile using busulfan-d8 as the internal standard. Separation was performed on a Kinetex C18 column (2.1 × 50 mm, 5 µm; Phenomenex) with isocratic elu...