Single-Objective Light-Sheet Microscopy Allows Long-Term, High-Resolution In Vivo Brain Imaging of Drosophila
In vivo imaging of dynamic subcellular brain structures is key to understanding several phenomena in neuroscience. However, in the fruit fly, its implementation has been hindered by a trade-off among spatial resolution, speed, photobleaching, phototoxicity, and setup complexity required to access specific target regi...