Carbene Footprinting and Mass Spectrometry Capture Global Conformational Changes in Elongation Factor-Tu Induced by Antibiotic Binding
Carbene footprinting methodology, in combination with mass spectrometry, was employed to study the interactions of bacterial elongation factor-thermo-unstable (EF-Tu) with two antibiotics. EF-Tu, a GTPase, delivers aa-tRNA to the ribosome during protein synthesis where it undergoes a significant conformational switch...