Author

G. Kim

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Open access Sep 2026

Programmable in vivo mRNA circularization for enhanced gene expression in bacteria

Abstract The minute-scale lifetime of mRNA strongly influences bacterial gene expression, whereas a robust and programmable approach to directly control the mRNA stability and topology remains elusive. Here, we develop CRESEnT (Circular RNA Expression for Stable and Enhanced Translation), a programmable in vivo mRNA ci...

Seongjun Park, G. Kim, Min Ha Kim et al. · Nucleic Acids Research · 0 citations

2026

Construction of a Tl-CRISPRi Genetic Circuit in Bacteria for Translation-Level Gene Knockdown.

This chapter describes how to design spacer sequences and install them into the guide RNA expression plasmid and describes how to mutate the handle of gRNA to achieve tunable knockdown of a target gene.

G. Kim, H. Kim, Sang Woo Seo · Methods in molecular biology · 0 citations