High-throughput development and scale-up of recombinant adeno-associated virus 5 anion-exchange chromatography for high-purity capsid separation.
A high-throughput, ultra-scale-down platform using 200 μL RoboColumns™ was established to enable rapid screening of salt systems, additives, and operating conditions with minimal material requirements, and the identification of a magnesium-only system that improved both enrichment and recovery.