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Sponsored Design Multi-Agent AI Systems Using MCP and A2A: Engineer your own Pyt Design Multi-Agent AI Systems Using MCP and A2A: Engineer your own Pyt

Author

Gibson Dowd

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Open access Jul 2026

Protocol for one-step design of a triple sgRNA-based CRISPR/Cas9 construct for neuronal gene knockout in the mouse brain

This protocol enables rapid, one-step assembly of triple-sgRNA expression cassettes and describes steps for CRISPR knockout sgRNA design, plasmid construction and verification, animal preparation, and neonatal adeno-associated viral vector delivery.

Gibson Dowd, Yuki Ogawa · 1 citation

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