Guang-Chao Wang

Rapid discovery of monoclonal antibodies via high-throughput single BCR affinity sequencing

Abstract To jointly capture antigen-binding affinity and paired heavy–light-chain sequences at scale has remained a bottleneck for monoclonal antibody discovery. Here, we present Antigen Affinity BCR-seq (AAB-seq), a high-throughput single-cell sequencing platform that can obtain the relative antibody–antigen affinity...