Engineering 2,4-Diacetylphloroglucinol-Hyper-Tolerant Escherichia coli via Adaptive Evolution for Efficient Biosynthesis and an Organic Solvent-Free Downstream Process.
2,4-Diacetylphloroglucinol (DAPG) is a valuable antimicrobial compound with significant agricultural potential, suffers bioproduction limitations from host toxicity and inefficient downstream processing. This study engineered DAPG-hyper-tolerant Escherichia coli via adaptive laboratory evolution (ALE) starting from a p...