Guinevere A. Ashley

An F2A sequence permits correct localization of a secreted and a nuclear localized reporter in C. elegans

Multicistronic expression systems enable production of multiple proteins from a single transcript, with internal ribosome entry sites (IRES), SL2 trans-splicing, and 2A peptides as common tools. Because 2A peptides rely on a single translation event, we tested whether nuclear-localized mStayGold (mSG::H2B) and secreted...