Expression of Soluble c-CfTXB Protein from Chironex fleckeri Jellyfish Fused to SUMO Tag in Escherichia coli BL21 (DE3) and Evaluation of Immunogenicity in Syrian Mice
Fusion of the SUMO tag to the engineered c-CfTXB protein improved its folding, expression yield, purification efficiency, and solubility, and the successful immunogenicity of the antigen in the mouse model may pave the way for the design of recombinant vaccines to neutralize box jellyfish venom.