Chemical Pathway-Dependent Structural Reorganization at a Hinge Microenvironment in the AcrA Adaptor Protein.
Adaptor protein AcrA plays a central role in the assembly and function of tripartite multidrug efflux pumps in Gram-negative bacteria, yet how its structural organization responds to coupled chemical perturbations rather than solely to equilibrium conditions remains unclear. Residues near His285 define a hinge microenv...