Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
INTRODUCTION Cell-free DNA (cfDNA) is a promising non-invasive biomarker of organ injury. In transplantation, cfDNA is typically quantified by distinguishing donor- from recipient-derived DNA. However, we aimed to develop a multiplex digital droplet PCR (ddPCR) assay to measure kidney-derived cfDNA independent of donor...