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Hai-Lin Yang

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Open access Sep 2026

Tn5-EGIM: efficient Tn5 transposon mutagenesis to identify non-essential bacteriophage genes for genome minimization and engineering

Generating high‑quality mutant libraries is a powerful strategy for systematic analysis of gene essentiality and function. However, current genomic tools perform poorly for bacteriophages (phages) due to a lack of efficient selectable markers. Here we show a Tn5 -mediated e ssential g ene i nsertion m uta...

Hai-Lin Zhang, Jia-Hui Sun, Hui-Lin Xu et al. · 0 citations

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