Hai-Lin Zhang

Tn5-EGIM: efficient Tn5 transposon mutagenesis to identify non-essential bacteriophage genes for genome minimization and engineering

Generating high‑quality mutant libraries is a powerful strategy for systematic analysis of gene essentiality and function. However, current genomic tools perform poorly for bacteriophages (phages) due to a lack of efficient selectable markers. Here we show a Tn5 -mediated e ssential g ene i nsertion m uta...