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Author

Haidong Huang

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Jul 2026

Computationally Guided Engineering of Multi-Enzyme Cascades Enables Efficient Trehalose Biosynthesis.

Trehalose is a nonreducing disaccharide widely used for its biomolecule-protective properties. However, multienzyme cascade production remains limited by low enzyme expression and suboptimal catalytic performance. To address this, thermostable maltooligosyltrehalose synthase (TreY) and trehalohydrolase (TreZ) from Arth...

Haidong Huang, Yang-Yang Li, Jin-Song Song et al. · 1 citation
Aug 2026

Mechanism-Guided Synergistic Engineering of Substrate Access and Catalytic Microenvironment in Oxidosqualene Cyclase for Enhanced Amyrin Biosynthesis.

Oxidosqualene cyclases (OSCs) catalyze the cyclization of 2,3-oxidosqualene into diverse triterpenoids, yet their intrinsically low catalytic efficiency restricts biosynthetic productivity. Here, we establish a mechanism-guided synergistic engineering strategy that extends beyond conventional active-site engineering by...

Yang-Yang Li, K. Jin, Jiangong Lu et al. · 0 citations

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