Searching for a Vanishing Fish: Development of an eDNA-Based Digital PCR Assay and Field Surveys for the Critically Endangered Chinese Bahaba
The critically endangered Chinese bahaba (Bahaba taipingensis) is difficult to monitor via conventional methods due to its low abundance and estuarine habitat. We developed a species-specific microfluidic digital PCR assay targeting the mitochondrial COI gene for environmental DNA (eDNA) detection. No cross-amplificati...