Sep 2026
Efficient Production of Vanillyl Alcohol in Escherichia coli via Protein and Pathway Engineering
Vanillyl alcohol (VNA) is a high-value compound used in the pharmaceutical industry and other fields. Its microbial de novo biosynthesis is severely limited by the low catalytic efficiency of O-methyltransferase. Here, we constructed a V314I mutant of caffeate O-methyltransferase via structure-guided protein engineer...
Jin-Yi Li, Yong Du, Jia Wang et al.
· ACS Synthetic Biology · 0 citations