PAM-Free Programmable Enzymatic Cascade Design Enabling Highly Sensitive CRISPR/Cas12a Lateral Flow Assay for Foodborne Pathogen Detection
CRISPR/Cas12a-assisted nucleic acid assays have shown great potential for rapid pathogen detection. However, their implementation in lateral flow assays remains constrained by strict protospacer adjacent motif (PAM) requirements and low-contrast, signal-off readouts. To effectively address these limitations, we prese...