Screening of mutants, enzymatic properties, and scale-up fermentation for catalysis of naringin using recombinant α-L-rhamnosidase
Background Prunin is scarce in nature and suffers from low extraction efficiency, and industrial-scale production of prunin and L-rhamnose remains technically inadequate. Therefore, developing an α-L-rhamnosidase capable of efficient and specific naringin hydrolysis is of great practical significance. Methods An α-L-rh...