Heman Kumar Ramiya Ramesh Babu

mCranberry: A rationally engineered monomeric red fluorescent protein with 85% quantum yield

A novel surface-mutation-guided engineering strategy combined with iterative rounds of rational mutagenesis to improve key photophysical properties of red fluorescent proteins (RFPs) and provides a framework that may be broadly applicable to other pigment-protein systems such as genetically encoded metal-ion indicators...