Integrating Morphology and Transcriptional Regulation to Alleviate Intracellular Product Stress for Enhanced Indigo Biosynthesis in Escherichia coli.
Indigo is an important biobased colorant used in textile dyeing and as a precursor for blue food colorants; however, its microbial production is often limited by cytotoxicity and intracellular accumulation of insoluble products. Here, we established a systems-level host engineering framework in Escherichia coli to impr...