Cryo-EM Structure of a Monomeric Safracin B Nonribosomal Peptide Synthetase Enabled by an MbtH-like Protein and Covalent Probe Trapping
Nonribosomal peptide synthetases (NRPSs) assemble structurally complex natural products but remain challenging targets for high-resolution structural analysis because of their conformational heterogeneity and dynamic catalytic cycles. SacC/SfmC is an unusual single-module NRPS that diverges from canonical peptide-for...