Rational Engineering of a Sucrose Phosphorylase for Switchable Regioselective Synthesis of Antioxidant Gallic Acid α-Glucosides.
Gallic acid is a bioactive polyphenol with poor solubility and stability. Enzymatic α-glucosylation can address these limitations, but regioselective synthesis among multiple phenolic hydroxyls remains challenging. Here, we engineer a sucrose phosphorylase from Bifidobacterium adolescentis (BaSP) for the switchable syn...