Enhancing the flexibility of the peripheral regions within the catalytic cavity of a family 5 glycoside hydrolase CelB for improving catalytic efficiency
Glycoside Hydrolase family 5 (GH5) endoglucanases are indispensable biocatalysts in lignocellulosic biorefining and their intrinsic catalytic performances play vital roles in process economics and enable broader industrial applicability. Herein, we engineered the catalytic domain of a bi-modular GH5 endoglucanase Cel...