This study aims to prepare specific monoclonal antibodies (mAbs) of IBDV VP3. We purified the recombinant VP3 protein to immunize the BALB/c mice for subsequently cell fusion. Following three rounds of sub-cloning, we successfully obtained three hybridoma cell lines (3B, 5 G, 6A) capable of stably secreting anti-VP3 mA...
Infectious bursal disease virus (IBDV) of the Birnaviridae family is a non-envelope, double-stranded RNA virus that encodes a VP3 protein with multiple functions, which controls viral genome replication, IFN-β production, and virus traffic in infected cells. Posttranslational modifications (PTMs), such as ubiquitinatio...
Avian reovirus (ARV) causes of multisystemic, immunosuppressive infectious diseases in poultry. Among them, the σC protein, a structural component of the outer capsid encoded by the S1 genome segment, plays a crucial role in viral attachment and cellular entry. In this study, we expressed the σC protein derived from th...
