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Open access Oct 2026

Rate-Limiting Enzyme Design in Propionaldehyde Oxidation-Driven Dual-Cofactor Regeneration System

In vitro biomanufacturing offers an efficient platform for green synthesis; however, simultaneous in situ regeneration of NADPH and ATP remains challenging. Herein, a three-enzyme cascade driven by propionaldehyde oxidation is constructed for dual-cofactor regeneration, comprising an engineered NADP+-preferring aldeh...

Ze-Bin Liu, Hui Ren, Yao-Yao Zhao et al. · 0 citations

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