Rate-Limiting Enzyme Design in Propionaldehyde Oxidation-Driven Dual-Cofactor Regeneration System
In vitro biomanufacturing offers an efficient platform for green synthesis; however, simultaneous in situ regeneration of NADPH and ATP remains challenging. Herein, a three-enzyme cascade driven by propionaldehyde oxidation is constructed for dual-cofactor regeneration, comprising an engineered NADP+-preferring aldeh...