The increasing prevalence of antibiotic-resistant bacteria has encouraged the exploration of natural sources of antibacterial compounds, including Tridax procumbens L. This study aimed to evaluate the antibacterial activity of leaf, stem, and flower extracts of T. procumbens against Staphylococcus aureus, Escherichia coli, and Pectobacterium sp. Plant samples were collected in Denpasar, Bali, dried, powdered, and extracted by maceration using 96% ethanol, n-hexane, and ethyl acetate. Antibacterial activity was assessed using the disc diffusion method at extract concentrations of 10–50%, with 1% DMSO as the negative control. Inhibition zone diameters were analyzed using analysis of variance followed by the least significant difference test at the 5% significance level. Leaf and stem extracts showed no antibacterial activity against any tested bacteria. Antibacterial activity was observed only in the flower extracts against S. aureus, with the n-hexane extract producing the largest inhibition zone (10.0 mm at 50% concentration), followed by the ethyl acetate extract, while the ethanol extract was inactive. No extracts inhibited E. coli or Pectobacterium sp. These findings indicate that n-hexane flower extract possesses limited antibacterial activity against S. aureus, highlighting its potential as a source of antibacterial compounds and the need for further purification and characterization of its bioactive constituents.
Yuliatin Rahman, I. K. Suada, I. Wijaya et al.· Research Horizon· 0 citations
Fusarium wilt disease caused by Fusarium oxysporum f. sp. melonis is a major factor reduces of melon (Cucumis melo L.) production in Indonesia. Control using chemical fungicides has not been effective and poses risks to the environment and health. The aim of this experiment was to find out more accurate treatment combination of Trichoderma spp. as biological control agents with neem leaf powder (Azadirachta indica) as a botanical fungicide for inhibiting the growth of F. oxysporum in vitro. The Trichoderma species used in this study were collected by the Plant Pathology Laboratory collection of Udayana University. The research was conducted using a Randomized Complete Design (RCD) with two factorial treatments comprising three Trichoderma species (T. harzianum, T. koningii, and T. viride) and four neem leaf powder concentrations (0 g L⁻¹, 15 g L⁻¹, 30 g L⁻¹, and 45 g L⁻¹) and 3 replications. Antagonistic activity was assessed using the dual culture method on potato dextrose agar (PDA) medium, with observations conducted over seven days observed parameters included pathogen colony growth and percentage of inhibition. The results showed that the combination of Trichoderma spp. and neem leaf powder significantly inhibited the growth of F. oxysporum in vitro, with varying effectiveness among Trichoderma species. The best inhibition was obtained from the combination of Trichoderma koningii and neem leaf powder at a concentration of 45 g L⁻¹, which completely inhibited the growth of F. oxysporum (100%) by day 5 without suppressing the growth of Trichoderma.
R. Pakpahan, I. K. Suada, T. A. Phabiola· International journal of mul...· 0 citations
We use cookies to run the site and, with your consent, for analytics and to show ads.
See our Cookie Policy.
