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I. Safenkova

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Open access Jul 2026

G-quadruplex folding uncouples cis-activation from collateral trans-cleavage by Cas12a

The CRISPR/Cas12a system is widely used in nucleic-acid diagnostics because Cas12a couples guide-directed recognition of a cis-target with indiscriminate collateral trans-cleavage of ssDNA. Controlling these activities through DNA structure can tune diagnostic signals. G-quadruplexes (G4s) are strong structural regulat...

I. Safenkova, Maria V. Kamionskaya, A. V. Samokhvalov et al. · 0 citations

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