Ilias G. Karvounis

A dual gating mechanism controls target‐strand cleavage in Cas12j: Implications for engineering efficient nickases

Abstract The rapid expansion of CRISPR technologies has unveiled a diverse repertoire of RNA‐guided endonucleases, among them the compact Cas12j, which has emerged as a promising genome‐editing tool. Cas12j cleaves the two DNA strands sequentially; however, the molecular mechanism that regulates this cleavage remains i...