Review
Jul 2026
Engineering T7 RNA Polymerase for High-Purity In Vitro Transcription
In vitro transcription using bacteriophage T7 RNA polymerase (T7 RNAP) is the gold-standard platform for RNA production in both research and therapeutic applications. Despite its high processivity and promoter specificity, T7 RNAP generates multiple RNA by-products, including double-stranded RNA, 3'-extended transcript...
Pauline Hermans, Y.I. Serikova, J. Castillo et al.
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