Author

J. Debler

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Open access Sep 2026

Make it so: Rapid and affordable plasmid sequencing on ONT platforms with PICARD-seq

Plasmid construction underpins molecular biology and synthetic biology, yet validation is often limited to the inserted fragment rather than the whole plasmid, and around a third of laboratory-made plasmids carry sequence errors that can affect function. Sanger sequencing scales poorly across whole plasmids, while shor...

Rebecca Wolters, J. Debler, Gabriella Go et al. · bioRxiv · 0 citations

Open access Sep 2026

Major effector loss reveals compensatory pathogenicity networks in a necrotrophic wheat pathogen

Necrotrophic effectors (NEs) are key determinants of virulence in the necrotrophic fungal pathogen Parastagonospora nodorum that causes septoria nodorum blotch of wheat. However, targeted removal of three important NEs SnToxA, SnTox1, and SnTox3 in the mutant Δtoxa13 previously revealed a redundancy mechanism is trigge...

S. Morikawa, C. Verdonk, Leon Lenzo et al. · bioRxiv · 0 citations