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Author

J. Gutbrod

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Open access Jul 2026

Engineering of T7 DNA-dependent RNA polymerase with activity at elevated temperature

Bacteriophage T7 RNA polymerase (T7 RNAP) is a key enzyme for in vitro transcription (IVT) and plays a central role in the production of synthetic mRNA for research and therapeutic applications. However, IVT frequently generates double-stranded RNA (dsRNA) as an undesired by-product, which can trigger innate immune res...

Svenja Hehn, J. Gutbrod, M. Gutjahr et al. · 0 citations

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