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J. M. Ragle

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Sep 2026

An F2A sequence permits correct localization of a secreted and a nuclear localized reporter in C. elegans

Multicistronic expression systems enable production of multiple proteins from a single transcript, with internal ribosome entry sites (IRES), SL2 trans-splicing, and 2A peptides as common tools. Because 2A peptides rely on a single translation event, we tested whether nuclear-localized mStayGold (mSG::H2B) and secreted...

J. M. Ragle, Guinevere A. Ashley, Jordan D. Ward · 0 citations

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