2026
Monitoring structural dynamics of nascent bacterial riboswitches responding to TPP within transcriptional complexes.
This chapter describes a single-molecule fluorescence resonance energy transfer (smFRET) approach to monitor cotranscriptional RNA folding within native transcription complexes to study cotranscriptional regulatory mechanisms taking place either in bacterial or eukaryotic elongation complexes.
Mirette Hashem, Patrick St-Pierre, Adrien Chauvier et al.
· Methods in Enzymology · 0 citations