Identification and validation of condition-specific candidate reference genes for accurate RT-qPCR normalization in acute and chronic methamphetamine-exposed cynomolgus monkeys.
Reverse transcription quantitative real-time PCR (RT-qPCR) is widely used to quantify gene expression, but its accuracy depends on appropriate normalization using stable reference genes (RGs). Because methamphetamine (METH) exposure induces widespread transcriptional changes, conventional housekeeping genes may not rem...