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Author

Jeanine F. Amacher

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Open access Aug 2026

A FRET Ligation Assay using Fluorescent Proteins for Bacterial Sortase Enzymes

Bacterial sortases are widely used in sortase-mediated ligation (SML) experiments for various protein engineering applications. The power of these enzymes to bind and cleave a specific recognition motif, followed by ligation to another substrate using a ping-pong reaction mechanism has numerous applications in vaccine...

Adam Wachsman, Erich G. Walkenhauer, Kaia Stover et al. · 0 citations
Open access Aug 2026

Viral PDZ target sequences are not inherently more promiscuous than those derived from endogenous target proteins

The data support previously reported sequence hotspots, whereby certain PBMs are recognized by large numbers of PDZ domains, likely due to a combination of widely favorable modulator residues, and support previously reported sequence hotspots.

Cole D. Masuga, Caroline Ceravolo, Elise F. Tahti et al. · 0 citations
Open access Aug 2026

Mutation-induced heterogeneity of the β7-β8 loop of the Staphylococcus aureus class A sortase leading to enhanced catalytic efficiency characterized by NMR and enzyme kinetics

NMR data, in combination with enzymatic assays using active variants confirmed differences in the active, apo states of these enzymes, and provided additional atomic detail regarding the importance of the P94 residue in saSrtA substrate recognition.

Erich G. Walkenhauer, Noah Cox-Tigre, M. Chaubey et al. · 0 citations

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