Open access
Sep 2026
A dynamically frustrated allosteric checkpoint consistent with conformational proofreading in CRISPR–Cas9
Abstract CRISPR–Cas9 DNA cleavage requires R-loop extension to activate the histidine–asparagine–histidine (HNH) nuclease domain, yet how heteroduplex maturation gates catalytic commitment remains unclear. Using extensive molecular dynamics simulations across seven experimentally trapped heteroduplex states (6–18 nt),...
Jian-Xiang Huang, Jia-Cheng Wei, Hua-Zhang Chen et al.
· Nucleic Acids Research · 0 citations