Jia Liu

[Efficient production of l- lysine by Escherichia coli through systems metabolic engineering].

Genome-scale overexpression screening identifies product tolerance and efflux transport as key determinants of high-level L-tryptophan production in Escherichia coli.

L-tryptophan is a high-value aromatic amino acid widely used in the food, feed, and pharmaceutical industries. However, large-scale microbial production is constrained by insufficient precursor supply and limited strain tolerance to high product concentrations. In this study, modular metabolic engineering was first emp...

Reprogramming energy system for growth and bioproduction from CO₂ and methanol in Escherichia coli

Microbial CO₂ assimilation provides a strategy for sustainable biomanufacturing. However, CO₂ reduction requires substantial energy input, while native energy systems are tightly regulated and extensively consumed by endogenous metabolism, limiting assimilation efficiency. Here, we design and construct an orthogonal en...

From laboratory to industry: Systematic approaches to the biomanufacturing of short-chain diols.

These analyses provide an evidence-based framework for product-specific pathway and process decisions in short-chain diol biomanufacturing and identify emerging opportunities for bioconversion and catalytic upgrading into high-value chemicals and functional materials.

Metabolic flux reprogramming and protein engineering drive efficient l-arginine biosynthesis.

An enzyme-constrained model was used to identify 11 gene targets affecting l-arginine production and engineered the rate-limiting enzyme argininosuccinate synthetase to construct the strain Arg11, achieving the best performance reported to date.