A One-Step Quadruplex RT-ddPCR Assay for Simultaneous Detection and Absolute Quantification of Respiratory Viruses in Wastewater from Multiple Settings
Wastewater-based surveillance of respiratory viruses increasingly requires multiplex assays capable of detecting low-abundance targets in complex environmental matrices without standard curves. In this study, a highly sensitive and specific one-step quadruplex reverse transcription droplet digital PCR assay was devel...