Geranylgeranyl diphosphate synthase (GGPS) is a key enzyme in terpenoid biosynthesis, but its role in Phoebe zhennan remains largely unknown. Here, 14 PzGGPS genes were identified and classified into three groups with conserved gene structures and motifs. Among them, PzGGPS12 was identified as a key gene for terpenoid biosynthesis, wood fragrance formation, and drought tolerance. Functional analyses showed that PzGGPS12 catalyzes the production of terpene precursors and promotes the accumulation of mono-, sesqui-, di-, and triterpenoids, thereby enhancing drought resistance through metabolic flux regulation. Furthermore, PzNAC6, PzWRKY12, and PzC3H8 were identified as major upstream transcription factors controlling the PzGGPS12 expression. These results reveal the regulatory network of PzGGPS12 and highlight its dual roles in wood fragrance formation and drought adaptation, providing new insights into terpenoid metabolism and molecular breeding of P. zhennan.
Jiang-Hong Qian, Xin Huang, Yun-Jie Gu et al.· Journal of Agricultural and...· 0 citations
Background: Natural hybridisation is prevalent among Populus species in the Hengduan Mountains. Clarifying their interrelationships and hybrid formation mechanisms is critical for taxonomic classification and breeding of the genus.
Methods: This study sequenced the complete chloroplast genomes of three Populus species – P. lasiocarpa Oliv. (Sect. Leucoides Spach), P. gonggaensis N. Chao & J.R. He (Sect. Leucoides Spach), and P. cathayana Rehd. (Sect. Tacamahaca Spach) - to investigate their genetic relationships and evolutionary patterns.
Results: The chloroplast genome lengths were 156,554 bp (P. lasiocarpa), 156,494 bp (P. gonggaensis), and 156,812 bp (P. cathayana), with all sharing a 37% GC content and conserved quadripartite structure (large/small single-copy regions plus two inverted repeats). They contained 130 or 131 genes (85 or 86 protein-coding, 37 tRNA, 8 rRNA) and 129-142 simple sequence repeat (SSR) loci (primarily adenine/thymine-dominated mononucleotide repeats). Phylogenetic analysis revealed the three species form a monophyletic clade, with P. gonggaensis and P. cathayana showing close affinity.
Conclusions: Chloroplast evidence indicated that P. gonggaensis is maternally closely related to P. cathayana, supporting its maternal lineage from P. cathayana. Further nuclear genome evidence is needed to confirm the hybrid origin of this species.
Liangxing Zhang, Jia Mi, Xue-Qin Wan et al.· New Zealand Journal of Fores...· 0 citations
Chalcone synthase (CHS) is a key enzyme in flavonoid biosynthesis, and its activity strongly influences the efficiency of flavonoid production in plants. By controlling the biosynthesis of secondary metabolites, CHS contributes to plant adaptation to diverse environmental stresses. Here, we identified CHS members across the Phoebe zhennan genome and systematically examined their gene structures, phylogenetic relationships, and potential roles in golden-thread wood color formation and drought response. Genome-wide screening resolved 11 putatively functional PzCHSs, which were unevenly distributed across six chromosomes. Physicochemical analyses indicated that most PzCHS proteins were hydrophilic. Phylogenetic reconstruction divided the PzCHS family into three groups, whose members generally shared similar gene structures. One tandem duplication event and five segmental duplication events were detected. Synteny analysis revealed extensive collinearity between the CHS families of P. zhennan, Phoebe bournei and Cinnamomum camphora, consistent with their close evolutionary relationships. Promoter regions of PzCHS genes were enriched for phytohormone-responsive and growth-related cis-elements, suggesting roles in development and hormonal regulation. Transcriptome profiling showed that PzCHS6 was upregulated in the transition zone of wood and under drought stress, suggesting that this gene may be associated with both golden-thread wood color formation and drought response. These results provide a framework for functional dissection of the CHS members and offer candidate genes for golden-thread wood color improvement and drought response breeding.
Kui Yan, Shuai He, Jian Peng et al.· Genes· 0 citations
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