A novel homozygous chromosomal deletion encompassing the ACTL9 gene is associated with cIVF polyspermy and male infertility.
A homozygous chromosomal microdeletion within the ACTL9 gene in an infertile male characterized by polyspermy in cIVF and nearly total fertilization failure in ICSI is identified and could facilitate the diagnosis of sperm-borne polyspermy.