Jing Ni

The effect of sedimentation on the unbound fraction of therapeutic peptides in plasma determined using ultra-centrifugation method.

Centrifugation is a critical step in determining the unbound fraction of drug (ƒu) in plasma, as it separates unbound drug molecules from protein-bound species during plasma protein binding (PPB) assays. When applied to therapeutic peptides, however, ultracentrifugation may induce vertical concentration heterogeneity w...