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Open access Aug 2026

Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins

ABSTRACT To overcome the labor‐intensive nature of traditional directed evolution, the promoted Escherichia coli‐assisted continuous evolution (PEACE) system has been developed. PEACE 1.0 employs a cytidine deaminase‑T7 RNA polymerase (T7 RNAP) fusion for targeted mutagenesis and integrates a toxin–antitoxin selection...

Xinyu Zhang, Zhan-Zhi Liu, Ying Xu et al. · 0 citations
Sep 2026

Cross-target and cell-preferential CRISPR-Cas9 inhibition with carbohydrate-tagged oligonucleotides.

Precise control of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) activity is important for limiting off-target effects and chromosomal rearrangements. Existing inhibitors, including anti-CRISPR proteins and spacer-targeting oligonucleotides, can be constrained by...

Bing-Zhi Li, Shu-Lin Wang, Min Cao et al. · 0 citations

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