Jing Yuan

Orthogonal Cas13a/Cas12a cascade for one-pot amplification-free detection of miRNA-21.

MicroRNAs (miRNAs) are promising biomarkers for clinical diagnosis and disease monitoring. However, current CRISPR/Cas-based miRNA sensors generally require reverse transcription or nucleic acid amplification to improve sensitivity, which complicates the workflow and increases the risk of contamination, nonspecific amp...